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Peer-reviewed veterinary case report

Isolation of enterotoxigenic Escherichia coli from a foal with diarrhea.

Journal:
Journal of the American Veterinary Medical Association
Year:
1989
Authors:
Holland, R E et al.
Affiliation:
Department of Large Animal Clinical Sciences
Species:
horse

Plain-English summary

A 3-day-old foal was found to have diarrhea, and tests showed that it had a specific type of bacteria called enterotoxigenic Escherichia coli, which can cause illness. Researchers identified this harmful bacteria by checking for certain features, like tiny hair-like structures (pili) and the ability to produce a toxin. They used special tests to confirm the presence of these features and ruled out other possible causes of the diarrhea. Based on their findings, they concluded that this particular strain of E. coli was the reason for the foal's diarrhea.

Abstract

Enterotoxigenic Escherichia coli was isolated from a 3-day-old foal with diarrhea. The isolate was distinguished from nonpathogenic E coli by determining the presence of pili and enterotoxin production. A standard slide agglutination test was performed, using pooled antisera that contained antibodies against K99 and F41 pilus antigens, K87 capsular antigen, and 0101 somatic antigen. Agglutination of the antisera occurred in the presence of the isolate. Piliation was verified by use of negative-contrast electron microscopy. Further, the isolate produced a heat-labile enterotoxin-like antigen that cross-reacted with a reagent containing formalin-treated, heat-killed Staphylococcus aureus (cowan 1 strain) bearing anti-cholera antibodies. On the basis of the aforementioned procedures and the absence of other identifiable enteric pathogens, we believe that E coli was responsible for causing diarrhea in the foal.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/2563724/