Investigation of the prion protein gene (PRNP) polymorphisms in Anatolian, Murrah, and crossbred water buffaloes (Bubalus bubalis).

Abstract

Bovine spongiform encephalopathy (BSE) of the cattle is the outstanding disease among other transmissible spongiform encephalopathy (TSEs). It can be transmitted from the cattle to a human and causes a new variant of the Creutzfeldt-Jakob disease (CJD). It is known that prion protein coding gene (PRNP) plays a major role in the TSE susceptibility or resistance in some species. Recent researches demonstrated that the insertion (in) and deletion (del) polymorphisms within promoter and intron 1 region of the PRNP related to BSE susceptibility in cattle. In contrast to cattle, BSE has never been reported in water buffalo; hence, PRNP polymorphisms may be an explanation for buffalo resistance to BSE. The aim of this study was to evaluate allele, genotype, and haplotype frequencies of the PRNP promoter and intron 1 insertion/deletion (indel) polymorphism in healthy Anatolian, Murrah, and Murrah × Anatolian crossbred buffaloes. According to our findings, there were no deletion alleles at two mentioned loci. All studied buffaloes were monomorphic and have carried in/in haplotypes which are considered as the most resistant genotype to BSE.