Investigation of the course of GFAP in blood in the initial 24 hours in rats subjected to minor head trauma.

Abstract

AIM: Aim of this study is to evaluate the diagnostic efficacy of Glial Fibrillar Acidic Protein (GFAP) particularly in minor head traumas. MATERIALS AND METHODS: 72 female and male, 3 month-old, Sprague Dawley rats were used in the study. The rats were divided into 9 groups. Following anesthesia, all rats were placed in prone position. A 10 mm long and 3 mm thick stainless steel metal disc was fixed onto the skull using dental paste in order to sustain a closed head trauma and evenly distribute the weight throughout the skull. After placing it under the metallic pipe arrangement over a height of 80 centimeters and fixing to make it constant, 50 g metallic discs were released by free fall, and the head trauma was sustained thanks to the gravity-generated force. Blood samples were collected from the rats under anesthesia for biochemical GFAP analysis 10 minutes after the trauma and in 1, 2, 3, 4, 5, 6 and 24 consecutive hours later. RESULTS: GFAP has a peak, and its peak level at hours 1 and 2 in rats subjected to a minor head trauma, with a slight decrease afterwards. CONCLUSIONS: GFAP is an important marker in determining the severity of traumatic brain injury.