Integrated multiomics analysis illuminates immune and metabolic mechanisms in red crucian carp upon intestinal infection with Aeromonas hydrophila L3-3.

Abstract

Aeromonas hydrophila, a major pathogenic bacterium, poses a significant threat to the health and welfare of aquatic organisms. Nonetheless, the immunometabolic characteristics of red crucian carp (RCC, Carassius auratus red var.) following intestinal infection remain largely uncharacterized. In this study, RCCs receiving A. hydrophila L3-3 infection showed elevated tissue injury and antioxidant disruption in intestine. Multiomics analyses indicated that calcium signaling pathway, MAPK signaling pathway and endocytosis were significantly associated with immune regulation of RCCs. Additionally, infection-induced oxidative and inflammatory responses were correlated with MAPK dysregulation, along with 9-oxoODE synthesis and δ-Tocotrienol catabolism. This finding may deepen our understanding of A. hydrophila-induced immunometabolic responses in intestine of RCCs and provide practical implications for the control of intestinal infection in aquaculture.