Peer-reviewed veterinary case report
Implication of Broadly Neutralizing Bovine Monoclonal Antibodies in the Development of an Enzyme-Linked Immunosorbent Assay for Detecting Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype O.
- Journal:
- Journal of clinical microbiology
- Year:
- 2019
- Authors:
- Cao, Yimei et al.
- Affiliation:
- Lanzhou Veterinary Research Institute · China
Plain-English summary
This study focused on improving the way we detect neutralizing antibodies, which help protect against foot-and-mouth disease (FMD), in cattle. Researchers created two special antibodies from blood samples of cows that had been vaccinated against different strains of the FMD virus. They then developed a new test called NA-ELISA, which is faster and easier to use than the traditional method. This new test was found to be very accurate, correctly identifying nearly all neutralizing antibodies present in vaccinated animals. The results suggest that this new test could effectively evaluate how well vaccinated cows are protected against various strains of the FMD virus.
Abstract
Vaccination with inactivated vaccines is still the main measure to control foot-and-mouth disease (FMD) in areas where the disease is endemic, and the level of neutralizing antibody in vaccinated animals is directly related to their protection against virus challenge. Currently, neutralizing antibody is mainly detected using the virus neutralization test (VNT) based on cell culture, which is laborious and time-consuming and requires restrictive biocontainment facilities. In this study, two broadly neutralizing antibodies (bnAbs), E46 and F128, were successfully produced using techniques for the isolation of single B cells from peripheral blood mononuclear cells (PBMCs) from bovines sequentially immunized with three topotypes of foot-and-mouth disease virus (FMDV) serotype O. Based on these bnAbs, a blocking enzyme-linked immunosorbent assay (ELISA) for detecting neutralizing antibodies (NA-ELISA) against FMDV serotype O was developed. The specificity and sensitivity of the test were estimated to be 99.21% and 100%, respectively. A significant correlation ( < 0.01) was observed between the NA-ELISA titers and the VNT titers for all sera from vaccinated animals and for all tested strains, suggesting that the NA-ELISA could detect neutralizing antibodies against FMDV serotype O strains of wide antigenic and molecular diversity and could be used for the evaluation of protective immunity.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31578261/