Imaging Strategies for Acupuncture Intervention in Alzheimer's Disease Model Mice.

Abstract

Alzheimer's disease (AD) is a neurodegenerative disorder primarily characterized by progressive cognitive dysfunction. One of its typical pathological features is the formation of senile plaques in the brain due to the deposition of β-amyloid (Aβ) proteins. Acupuncture has demonstrated potential in clinical practice for improving cognitive function in AD patients. However, its dynamic effects on Aβ pathology require objective elucidation through modern technological approaches. Micro-positron emission tomography (micro-PET) provides a powerful tool for in vivo, non-invasive observation of Aβ deposition in the brains of AD model mice. In this protocol, we detail an imaging strategy utilizing [18F]AV-45 micro-PET to assess the effects of electroacupuncture intervention, complemented by Morris Water Maze behavioral testing and Western blot molecular analysis. The integrated approach enables the visualization and quantification of Aβ pathological progression in the brains of AD model mice. This provides objective and quantitative evidence to explore the regulatory effect of acupuncture on Aβ deposition. By integrating traditional Chinese medicine principles with modern molecular imaging and molecular biology technologies.