Histopathological, immunohistochemical and physiological study for the hepatoprotective effect of melatonin against inhrifampicin-induced hepatotoxicity in mice model.

Abstract

OBJECTIVE: Aim: The purpose of this study is to assess the hepatoprotective ef f ect of melatonin against isoniazid (INH) and rifampicin (RMP) induced hepatotoxicity in albino mice. PATIENTS AND METHODS: Materials and Methods: Adult male mice were divided into four groups: saline, INH-RMP, INH-RMP+MT and MT were administered for 21 days. Biochemical analyses were performed for the determination of ALT, AST. Histopathological changes in the liver and Immunohistochemical assessment to determine the expression of Caspace3 were also examined. RESULTS: Results: Biochemical analysis revealed signif i cant increases in serum ALT and AST in INH-RMP group. Histopathological fi ndings demonstrated severe liver damage in INH-RMP group as compared with control group. In contrast, treatment of mice with melatonin (MT) markedly mitigated the liver injury. Immunohistochemical fi ndings demonstrated apoptotic marker caspace3 signif i cantly higher in INH-RMP group as compared with control group. CONCLUSION: Conclusions: Experimental fi ndings highlight the potential benef i ts of melatonin in this model, prompting speculation on its potential application in human therapy.