Genomic Features of Antimicrobial Resistance inIsolated from Dogs with Pyoderma in Argentina and the United States: A Comparative Study.

Abstract

is the most common opportunistic pathogen in dogs and methicillin resistance (MRSP) has been identified as an emerging problem in canine pyoderma. Here, we evaluated the antimicrobial resistance (AMR) features and phylogeny ofisolated from canine pyoderma cases in Argentina (= 29) and the United States (= 29). 62% of isolates showed multi-drug resistance. The AMR genes found:A,Z,,G,A,M,(6')-(2″), in addition toK andA (only found in U.S. isolates). Two point mutations were detected:A(S80I)-A(S84L), andA(D84N)-A(S84L) in one U.S. isolate. A mutation inB (H481N) was found in two isolates from Argentina. SCCtype III, SCCtype V, ΨSCCwere identified in the Argentinian isolates; and SCCtype III, SCCtype IVg, SCCtype V, and SCCtype VII variant in the U.S. cohort. Sequence type (ST) ST71 belonging to a dominant clone was found in isolates from both countries, and ST45 only in Argentinian isolates. This is the first study to comparatively analyze the population structure of canine pyoderma-associatedisolates in Argentina and in the U.S. It is important to maintain surveillance onpopulations to monitor AMR and gain further understanding of its evolution and dissemination.