Genome-wide long non-coding RNA expression profile and its regulatory role in the ileocecal valve fromsubsp.-infected cattle.

Abstract

Bovine paratuberculosis (PTB) is a chronic enteritis caused bysubsp.(MAP), which results in significant economic losses to the dairy industry worldwide. Long non-coding RNAs (lncRNAs) play a crucial role in regulating the host immune response due to their interaction with transcripts in proximity. However, their annotation in cattle remains limited, and their role in cattle naturally infected with MAP has not been fully explored. In this study, lncRNAs were identified in the transcriptome of ileocecal valve samples from control cows without lesions ( = 4) and with PTB-associated focal ( = 5) and diffuse ( = 5) lesions in intestinal tissues using RNA sequencing. The raw reads were uploaded into the CLC Bio Genomics Workbench, and the trimmed reads were mapped to theARS_UCD1.2.109 reference genome using the Large Gap Read Mapping tool. The resulting annotation allowed the identification of 1,434 LncRNAs, 899 of which were novel, using the FlExible Extraction of LncRNA pipeline. LncRNA differential expression (DE) analysis performed withallowed the identification of 1, 6, and 2 DE lncRNAs in the comparisons of cows with focal lesions() controls, diffuse lesionscontrols, and diffusefocal lesions, respectively. Best lncRNA partner analysis identified expression correlations between the, lncRNA, and, and the(),and the(), respectively. Thenegatively regulates apoptosis, thepositively regulates IL-6 expression, and theregulates cell differentiation and inflammation. The results of the quantitative trait locus (QTL) enrichment analysis showed that the DE lncRNAs were located in genomic regions previously associated with clinical mastitis, HDL cholesterol, bovine tuberculosis, paratuberculosis, and bovine leukosis susceptibility. The identified DE lncRNAs could allow the development of novel PTB diagnostic tools and have potential applications in breeding strategies for PTB-resistant cattle.