First Whole Genome Sequencing of Crimean-Congo Hemorrhagic Fever Virus (CCHFV) in Tick Species within Ghana.

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a serious viral zoonotic disease spread by ticks and caused by the Crimean-Congo hemorrhagic fever virus (CCHFV). The emergence and reemergence of CCHF in various nations in the Eastern Mediterranean Region over the last decade have shown a growing risk of the disease spreading to new areas, especially in population-dense and livestock trade-dominant areas. There is a lack of updated information on the risk of CCHFV in the Greater Accra and Upper East Regions of Ghana. Due to the paucity of available data, this study sought to identify the tick species diversity in Ghana and to ascertain the CCHFV strains they may carry. A total of 705 ticks were collected from 188 cattle and 11 horses and morphologically identified. Three tick genera (,, and) were observed, with the predominant species being( = 290, 41.1%). The CCHFV infection rates of 0.78%, 0.69%, and 0.64% were recorded in,, and, respectively. No infection was detected in thespecies. Furthermore, a strain was successfully recovered using next-generation sequencing. The strain belongs to genotype 3 and shared 98.9% nucleotide identity with DQ211641_Mauritania_1984 and MF287636_Spain_2016. Findings from this study suggest the possible importation of the virus into the country through trade, and potentially, a public health threat to humans who may have primary contact with livestock.