First detection and genomic analysis of-positiveInfantis isolated from a broiler production system in the United Arab Emirates.

Abstract

This study reports the first detection of-mediated colistin resistance inserovar Infantis from a commercial broiler farm in the United Arab Emirates (UAE). Twoisolates (SAL_93 and SAL_94) were recovered from caecal droppings and characterized using whole-genome sequencing (WGS). Genomic analysis revealed a single-nucleotide polymorphism (SNP) difference between them, confirming their close epidemiological relationship. Both isolates belonged to multilocus sequence type 32 and exhibited multidrug resistance (MDR), including resistance to colistin (MIC = 4 mg/L) and ciprofloxacin (MIC = 0.5 mg/L). Notably, thegene was detected on a conjugative IncX4 plasmid. Additionally, the isolates harbored a large (275,043 bp) conjugative IncFIB plasmid carrying multiple AMR genes, including,,,. Bioinformatic analysis showed a high identity for globally reported-carrying IncX4 plasmids. The investigation of virulence-associated factors in the studied isolates identified 162 potential virulence-related genes. These included genes linked to the type 3 secretion system, specifically those encoded by pathogenicity island-1 (SPI-1). However, multiple genes linked to the second type 3 secretion system, encoded by SPI-2, were absent in all isolates. These findings suggest a potential risk of horizontal gene transfer in poultry production. Given these risks, the UAE's recent ban on colistin in veterinary medicine marks a crucial step in mitigating AMR transmission within a One Health framework.