Fermentedflavonoids: anti-pseudorabies virus efficacy and mechanismsand.

Abstract

INTRODUCTION: exhibits anti-inflammatory properties, protects neuronal cells and enhances immune function. Modern traditional Chinese medicine fermentation techniques can increase the bioactive compound content in. However, its potential therapeutic effects against the porcine pseudorabies virus (PRV) remain unclear. METHODS: A PRV infection model was established in mouse trigeminal ganglion (TG) cells to determine the optimal antiviral mode of action of flavonoids from fermented(FEBF). Additionally, a PRV-infected rat model was developed to evaluate theantiviral efficacy of FEBF. RESULTS: FEBF demonstrated a higher protective rate and a lower viral copy number compared to unfermentedflavonoids (EBF). The protective effect was most pronounced under toxicological and inhibitory conditions, surpassing the blocking effect. PRV infection upregulated TLR4, MyD88, and NF-κB p65 protein expression during the pre-infection phase, followed by their downregulation after 12 h. FEBF regulated PRV-induced changes in protein expression, restoring them to near-normal levels by 36 h.assessments of pathological injury, PRV viral load, neuronal count, and neuronal apoptosis indicated that FEBF provided superior neuroprotection compared to both Minocycline (MINO), a broad-spectrum neuroprotective drug, and unfermentedMechanistic studies further revealed that FEBF modulated microglial polarization and regulated the inflammatory cytokines IL-6, TNF-, IL-4, and IL-10. CONCLUSION: These findings demonstrate that FEBF exhibits significant antiviral effects against PRV in bothandmodels. FEBF represents a promising candidate for the development of anti-PRV therapeutics.