Expression of molecular markers in blood of neonatal foals with sepsis.

Abstract

OBJECTIVE: To determine gene expression of selected molecular markers (tumor necrosis factor [TNF]-alpha, interleukin [IL]-1beta, IL-6, IL-8, IL-10, procalcitonin [PCT], and transforming growth factor [TGF]-beta) in the blood of healthy and sick foals. ANIMALS: 28 sick foals without sepsis, 21 foals with sepsis, and 21 healthy foals. PROCEDURES: Total RNA was extracted from blood samples and converted into complementary DNA (cDNA). Gene expression was measured for the molecular markers by use of real-time PCR assay, and final quantitation was performed with the comparative threshold cycle method. RESULTS: Samples from all foals yielded transcription for all markers. Expression of TNF-alpha and TGF-beta was significantly lower and that of IL-8 significantly greater in the sick-nonseptic and septic groups, compared with the healthy group. No significant difference in expression of IL-1beta, IL-6, and PCT was found between the healthy group and the 2 sick groups. Expression of IL-10 was significantly greater in nonsurvivors, compared with survivors. CONCLUSIONS AND CLINICAL RELEVANCE: The cytokine profile in foals with sepsis may suggest an immunosuppressive state. Expression of IL-10 may be a marker for identification of foals with a guarded prognosis.