Exploring the obesity-lung inflammation connection: DNAJA1 in focus.

Abstract

BACKGROUND: The association between obesity and pulmonary inflammation remains inadequately understood. This study investigates this connection within the U.S. population utilizing data from the National Health and Nutrition Examination Survey (NHANES) (https://www.cdc.gov/nchs/index.htm) spanning 2007 to 2012, aiming to identify potential biomarkers. METHODS: A cohort analysis of the NHANES database was conducted to explore the relationship between obesity and pulmonary inflammation. Differential expression analysis was carried out on two datasets of lung tissue from obese mice (GSE16377 and GSE38093). Key genes were further validated using a single-cell dataset (GSE216695) and mouse models of obesity and weight loss. RESULTS: The analysis of data from 6,644 participants confirmed a significant association between obesity and pulmonary inflammation. Five genes (Dnaja1, Hspd1, Hspa1a, Hsph1, and Stip1) were pinpointed through differential expression analysis of the GEO datasets. Single-cell analysis revealed distinct variations in the expression of Dnaja1 in natural killer (NK) cells and cDCs. Histological examinations of mouse tissues indicated elevated levels of Dnaja1 in the obese group, with levels normalizing in the weight-loss group. CONCLUSION: By synthesizing findings from cohort studies, single-cell data, and mouse models, this research delineates the relationship between obesity and pulmonary inflammation, identifying Dnaja1 as a potential biomarker associated with NK and DC cells.