evaluations of-fermentedpolysaccharides on alleviating food allergy activity.

Abstract

In order to explore theanti-food allergy activity ofsubsp.-fermentedpolysaccharides F1-ESP-3, an ovalbumin (OVA)-induced food allergy mouse model was established by ascites immunization and gavage. The weight, temperature, incidence of diarrhea, levels of allergic mediators and inflammatory factors in the serum of mice were analyzed. We analyzed the differentiation of mouse spleen lymphocytes and the proportion of sensitized mast cells by flow cytometry. The intestinal barrier status of mice was analyzed by intestinal pathological tissue sections and microbiota sequencing. The results showed that F1-ESP-3 could alleviate the food allergy symptoms of mice, such as hypothermia and loose stool; levels of OVA-specific immunoglobulin E, mast cell protease and histamine in the serum of sensitized mice and the proportion of dendritic cells and mast cells in mouse spleen were significantly reduced; in addition, F1-ESP-3 may protect the intestinal barrier and further improve the intestinal microenvironment of food-allergic mice by regulating the abundance ofand. F1-ESP-3 can further improve the intestinal microenvironment of food-allergic mice by upregulating the levels of, and may affect the signal pathways such as NOD-like receptor, MAPK, I kappa B and antigen processing and presentation.