Evaluation of dipstick-ELISA using 28 kDa Fasciola gigantica cathepsin l cysteine proteinase (FgCL3) for serodiagnosis of fasciolosis in naturally infected goats.

Abstract

In the present investigation 100 goats presented for slaughter were used to evaluate the 28kDa cathepsin l cysteine proteinase (FgCL3) dipstick-ELISA for the diagnosis of fasciolosis. Presence of Fasciola gigantica worms in liver at the time of slaughter was taken as a gold standard for the evaluation of the assay. Faeces, blood and liver were taken from all slaughtered goats. Biochemical parameters and coprological examinations using sedimentation technique were also taken into consideration. Total serum proteins, albumin, mean values of aspartate aminotransferase and alkaline phosphatase of positive group were not significantly different from those of the negative group. The diagnostic sensitivity of dipstick-ELISA was higher than coprological detection of fluke eggs. However, specificity was equal for both the tests. The accuracy of dipstick-ELISA in detecting circulatory antibodies during the course of disease was higher than coproscopic examination. In conclusion, the FgCL3 antigen dependent dipstick-ELISA was more reliable and has better scope for field application in the endemic areas.