Establishment of a secondary infection model of severe acute pancreatitis in swine.

Abstract

OBJECTIVE: Develop a swine model of secondary infection in severe acute pancreatitis (SAP). METHODS: Twenty-seven female swine were divided into 3 groups (1-3). In the first experiment, a SAP model was developed by retrograde injection of sodium taurocholate and trypsin into the pancreatic duct. In the second experiment, the SAP model was used to develop a secondary infection model. In groups 1 to 3, 10&#x2078;/mL or 10&#x2074;/mL Escherichia coli or saline were respectively used to inoculate necrotic areas of the pancreas using computed tomographic guidance. Biochemical, histopathological, and imaging analyses were used to characterize disease presentation. RESULTS: The survival rate was 85.2% (23/27) during the course of the 9-day experiment. The secondary infection rates in groups 1 to 3 were 100% (8/8), 37.5% (3/8), and 14.3% (1/7), respectively. In group 1, the infection rate was significantly higher in comparison to the other 2 groups (&#x3c7;&#xb2;=4.66 and 8.14, respectively, and both P<0.05). The biochemical and histopathological parameters and computed tomographic images indicated successful development of the SAP secondary infection model. CONCLUSIONS: The swine model of SAP secondary infection was successfully developed using a 2-step method, which could serve as a platform for SAP studies that need complex experimental manipulations for longer time spans, especially for imaging research.