Peer-reviewed veterinary case report
Establishment of a chronic restraint stress model of depression with C57BL/6J mice.
- Journal:
- Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
- Year:
- 2015
- Authors:
- Sun, Nan-nan et al.
- Affiliation:
- Department of Biochemistry and Molecular Biology · China
- Species:
- rodent
Abstract
OBJECTIVE: To establish a chronic restraint stress (CRS) model of depression,using C57BL/6J mice. METHODS: Totally 20 C57BL/6J mice were screened by weight,sucrose preference,and the results of open-field test. Then,they were equally randomized into two groups:normal control (NC) group and CRS group. Mice in the CRS group were under restraint 4 hours a day and their behavioral changes were evaluated after 21 days. RESULTS: The immobility time was significantly longer in CRS mice [(131.70 ± 21.65) s] compared with controls [(68.88 ± 8.43) s] (P=0.0304). CRS mice showed a significant decrease in sucrose preference during the time 0-24 h [(66.21 ±3.24)% vs.(79.46 ± 3.85)%, P=0.0196] and 0-48 h [(73.25 ± 1.50)% vs.(80.20 ± 2.26)%, P=0.0248] compared with controls. CONCLUSION: The C57BL/6J mice CRS models of depression were successfully established.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25676263/