Epidemiological characteristics of human- and chicken-derived CTX-M-type extended-spectrum β-lactamase-producing Escherichia coli from China.

Abstract

Bacterial resistance to β-lactams is mainly attributed to CTX-M-type extended-spectrum β-lactamases (ESBLs). However, the predominant sequence type (ST) of bla-carrying Escherichia coli (bla-Ec) in chickens, an important food animal, in China and its contribution to human β-lactam resistance are not investigated. In this study, approximately 1808 chicken-derived strains collected from 10 provinces from 2012 to 2020 were screened for bla-Ec, and 222 bla-Ec were identified. Antimicrobial susceptibility tests, whole genome sequencing and conjugation experiment were performed. All quality-controlled 136 chicken-derived bla-Ec and 1193 human-derived bla-Ec genomes were downloaded from NCBI and EnteroBase to comprehensively analyze the prevalence of bla-Ec in China. bla(153/358, 42.7% in chicken isolates; 312/1193, 26.2% in human isolates) and bla(92/358, 25.7% in chicken isolates; 450/1193, 37.7% in human isolates) were dominant in bla-Ec. The STs of bla-Ec were diverse and scattered, with ST155 (n = 21) and ST152 (n = 120) being the most abundant in chicken- and human-derived isolates, respectively. Few examples indicated that chicken- and human-derived bla-Ec have 10 or less core genome single nucleotide polymorphisms (cgSNPs). Genetic environment analysis indicated that ISEcp1, IS26 and IS903B were closely associated with blatransfer. The almost identical pc61-55 and pM-64-1161 indicated the possibility of plasmid-mediated transmission of blabetween humans and chickens. Although the genomes of most bla-Ec isolated from chickens and humans were quite different, the prevalence and genetic environment of blavariants in both hosts were convergent. CTX-M-mediated resistance is more likely to spread through horizontal gene transmission than bacterial clones.