Encephalitozoon and Enterocytozoon (Microsporidia) spores in stool from pigeons and exotic birds: microsporidia spores in birds.

Abstract

Microsporidia are considered to be a cause of emerging and opportunistic infections in humans, and the species that infect humans can also infect a wide range of animals, raising concerns for zoonotic transmission. To understand the role of birds in the transmission of diseases caused by microsporidia, we examined 196 fecal specimens from birds, including birds of the families Psittacidae, Emberizidae, Icteridae and Columbidae, using Gram-chromotrope stain and polymerase chain reaction (PCR). Of the 196 fecal samples surveyed, 48 (24.5%) tested positive for microsporidia. The prevalence of microsporidia infection was higher in pigeons (31.1%) than in other birds (18.8%). The species of microsporidia that were detected in the birds surveyed in this study included Encephalitozoon hellem (found in 16.3% of positive samples), Enterocytozoon bieneusi (5.6%), Encephalitozoon intestinalis (1.5%) and Encephalitozoon cuniculi (1%). All the internal transcribed spacer (ITS) sequence of the rRNA from the study samples matched (with 100% identity) their correlate reference genotypes in GenBank, which included E. hellem 1A (AF338367), E. hellem 3 (AF110328), E. cuniculi I (AF338410) and E. bieneusi EpbA (AF076040). No fecal sample contained more than one type of microsporidian species. This study implicates exotic birds and pigeons as potential sources of microsporidia infection for humans living in urban areas.