Effects of Ursolic Acid on Immune Function and Antioxidative Capacity in Weaned Rabbits.

Abstract

This study aimed to investigate the effects of dietary supplementation with different levels of ursolic acid (UA) on the growth performance, immune function, intestinal antioxidant capacity, and anti-inflammatory responses of weaned rabbits. A total of 160 Hyla meat rabbits aged 35 days were randomly assigned to four groups. Each treatment group consisted of 8 replicates, with 5 rabbits per replicate. The rabbits were fed a basal diet (control group, CON) or experimental diets supplemented with 50, 100, or 200 mg/kg UA for 28 days. Dietary supplementation with 50 mg/kg UA significantly increased (<i>p</i> < 0.05) the average daily gain and average daily feed intake. The villus height, crypt depth, and villus height to crypt depth ratio exhibited quadratic responses (<i>p</i> < 0.05) to increasing dietary UA levels, with rabbits fed 50 mg/kg UA showing optimal ileal morphology. Compared with the CON group, dietary supplementation with 50 mg/kg UA significantly enhanced (<i>p</i> < 0.05) cecal catalase activity, secretory immunoglobulin A, and interleukin-10 (IL-10) levels, while the addition of 200 mg/kg UA increased (<i>p</i> < 0.05) serum catalase activity. The concentrations of serum tumor necrosis factor-α (TNF-α) and cecal IL-10 responded quadratically (<i>p</i> < 0.01 and <i>p</i> = 0.01, respectively) as the dietary UA level increased. With increasing UA supplementation, cecal Kelch-like ECH-associated protein 1 and <i>IL-10</i> mRNA expression showed linear upregulation (<i>p</i> < 0.05), whereas nuclear factor erythroid 2-related factor 2 (<i>Nrf2</i>), superoxide dismutase 1 (<i>SOD1</i>), quinone oxidoreductase 1 (<i>NQO1</i>), TNF-α, interleukin-6, and interleukin-8 displayed quadratic responses (<i>p</i> < 0.05). Dietary UA at 50 mg/kg significantly downregulated cecal <i>TNF-α</i> and interleukin-1β mRNA expression while upregulating <i>Nrf2</i>, <i>NQO1</i>, and <i>SOD1</i> mRNA levels (<i>p</i> < 0.05). In conclusion, dietary supplementation with 50 mg/kg UA significantly improved the growth performance of weaned rabbits by improving intestinal morphology, immune function, and antioxidant and anti-inflammatory capacities, demonstrating its efficacy as a natural phytogenic feed additive.