Effect of body weight in the pathogenesis of ligature-induced periodontal disease in Wistar rats.

Abstract

OBJECTIVE: The aim of this study was to compare ligature-induced alveolar bone loss between obese and non-obese rats. MATERIAL AND METHODS: Thirty female Wistar rats were randomly divided into two groups: a test group comprising 14 rats fed with a "cafeteria diet" for 120 days in order to gain weight and a control group comprising 16 regularly fed rats. Ligatures were placed around the 2nd upper molars, and the contralateral teeth served as intra-group controls. After 30 days, the animals were killed and the maxillae were removed. Sodium hypochlorite was used to prepare the specimens, and the cementum-enamel junction was stained with methylene blue 1%. Morphometric analysis of alveolar bone loss was by standardized digital photographs and the distance between the cementum-enamel junction and the alveolar bone crest was measured using the software Image Tool 3.0. RESULTS: Body weight differed statistically between test and controls (268.6 and 242.4 g, respectively). Test animals demonstrated a mean (SD) alveolar bone loss of 0.51 (0.11) mm and in the controls 0.52 (0.14) mm in teeth with ligatures. No statistically significant differences were observed (ANOVA+Tukey), except for teeth with and without ligatures in both groups. CONCLUSIONS: The establishment and progression of alveolar bone loss in rats was not influenced by body weight in the present study.