Diagnostic value of hypoxia-inducible factor 1 alpha and adrenomedullin in acute mesenteric ischemia.

Abstract

BACKGROUND: This study aims to evaluate the diagnostic significance of adrenomedullin and hypoxia-inducible factor 1 alpha (HIF1-alpha) levels in an animal model of acute mesenteric ischemia. MATERIALS AND METHODS: In our study, a total of 21 male New Zealand rabbits were used, and the animals were divided into three groups for the study. Blood samples were taken for adrenomedullin and HIF1-alpha levels at 0, 1, 3, and 6&#xa0;h from the control, sham, ischemia groups. RESULTS: HIF levels of the ischemia group at the 3rd and 6th hours were statistically significantly higher than those in the sham (p&#x2009;=&#x2009;0.002, p&#x2009;<&#x2009;0.001, respectively) and control groups (p&#x2009;=&#x2009;0.002, p&#x2009;<&#x2009;0.001, respectively). Adrenomedullin levels of the ischemia group at the 1st, 3rd, and 6th hours were statistically significantly higher than those in the sham (p&#x2009;=&#x2009;0.004, p&#x2009;<&#x2009;0.001, p&#x2009;<&#x2009;0.001, respectively) and control groups (p&#x2009;<&#x2009;0.001, p&#x2009;<&#x2009;0.001, p&#x2009;<&#x2009;0.001 respectively). CONCLUSION: In this experimental model of acute mesenteric ischemia, hypoxia-inducible factor-1 alpha and adrenomedullin levels were significantly higher in the ischemia group compared with the control and sham groups. Adrenomedullin demonstrated an earlier increase, while hypoxia-inducible factor-1 alpha increased significantly at later time points. These biomarkers may provide supportive diagnostic value when interpreted alongside clinical and imaging findings; however, further clinical studies are required to confirm their role in the early diagnosis of acute mesenteric ischemia.