Peer-reviewed veterinary case report
Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction.
- Journal:
- Journal of feline medicine and surgery
- Year:
- 2007
- Authors:
- Pinches, Mark D G et al.
- Affiliation:
- University of Bristol · United Kingdom
- Species:
- cat
Abstract
In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16861024/