Peer-reviewed veterinary case report
Development of a reverse-transcription loop-mediated isothermal amplification method for detection of rabbit hemorrhagic disease virus.
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- Yuan, Dongwei et al.
- Affiliation:
- Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences · China
- Species:
- rabbit
Plain-English summary
Rabbit hemorrhagic disease virus (RHDV) is a serious virus that can cause severe liver damage in rabbits and has a high death rate. Researchers have developed a new testing method called RT-LAMP, which can quickly and accurately detect this virus. This method uses specific genetic markers from the virus and can show results in about 40 minutes, either through a simple visual test or under special light. It can detect very small amounts of the virus and has been successful in identifying different strains from infected rabbits in China. Overall, this new test could be a helpful tool for quickly spotting RHDV infections in rabbits.
Abstract
Rabbit hemorrhagic disease virus (RHDV) causes haemagglutination and severe liver damage, with a high mortality rate. To develop a rapid and sensitive method for the surveillance of RHDV, a one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was established using a set of four primers specific for the VP60 gene segment of RHDV. The established assay was performed at 64°C for 40 min under isothermal conditions, and the results were visualized directly by electrophoresis or as fluorescent signals under ultraviolet light. The detection limit of the RT-LAMP assay was 10 copies of viral RNA per reaction, which was comparable to quantitative real-time RT-PCR, and 100-fold more sensitive than standard RT-PCR. Furthermore, seven viral RNAs of field isolates in China could be detected successfully using this assay. Overall, the newly established RT-LAMP assay indicates the potential usefulness of the technique as a simple, rapid and sensitive procedure, and can visually detect RHDV infection without the need for any specialized equipment.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23178586/