Peer-reviewed veterinary case report
Development of a nested PCR assay to detect equine infectious anemia proviral DNA from peripheral blood of naturally infected horses.
- Journal:
- Archives of virology
- Year:
- 2012
- Authors:
- Dong, Jian-Bao et al.
- Affiliation:
- Department of Veterinary Microbiology · Japan
- Species:
- horse
Plain-English summary
Equine infectious anemia (EIA) is a serious disease that affects horses and can lead to significant losses in the horse industry. Researchers have developed a new testing method called a nested PCR assay, which can detect the virus's genetic material in the blood of horses that are naturally infected. This new method is more effective than previous tests that were based on lab-grown virus strains and can accurately identify the virus in wild horse populations. The new test has shown to be very reliable, matching traditional testing methods perfectly. This advancement offers a strong new option for managing and controlling EIA in horses.
Abstract
Equine infectious anemia (EIA) has posed a major challenge and caused significant losses to the equine industry worldwide. PCR detection methods have considerable potential as an adjunct to conventional serological diagnostic techniques. However, most published PCR methods, including that recommended by the OIE, were designed using laboratory-adapted virus strains and do not function with field isolates of EIA virus (EIAV). In the present study, a nested PCR assay for detection of EIAV proviral DNA in peripheral blood cells of naturally infected horses was developed. Primer sets were designed based on conserved 5' regions of the viral genome extending from the LTR to the tat gene. Preliminary studies demonstrated that the method has a detection limit of 10 genomic copies and, when applied to a naturally EIAV-infected feral horse population, shows 100 % correlation with conventional serological diagnostic techniques. This assay provides a powerful new tool in the control of EIAV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22798044/