Peer-reviewed veterinary case report
Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus.
- Journal:
- Journal of virological methods
- Year:
- 2015
- Authors:
- Yuan, Wanzhe et al.
- Affiliation:
- College of Animal Medicine · China
Plain-English summary
Researchers have developed a new testing method to detect porcine epidemic diarrhea virus (PEDV), which is a serious virus that affects pigs and can cause vomiting, diarrhea, and dehydration. This new test, called a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay, is much more sensitive than traditional testing methods, being able to detect very small amounts of the virus without confusing it with other viruses. The study showed that this new test could help diagnose PEDV infections more accurately in both clinical settings and on farms. Overall, the new nanoPCR assay is a promising tool for better managing and monitoring this virus in pigs.
Abstract
Porcine epidemic diarrhea virus (PEDV) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. In this study, a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay targeting the N gene of PEDV was developed and the sensitivity and specificity were investigated. Under the optimized conditions for detection of PEDV RNA, the nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay. The lower detection limit of the nanoPCR assay was 2.7 × 10(-6) ng/μL of PEDV RNA and no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nanoPCR assay for the detection of PEDV. The sensitive and specific nanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of PEDV-infection.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25887451/