Development and Standardization of Sandwich ELISA for the Detection of Canine Parvovirus Infection

Abstract

Canine parvovirus (CPV) is a highly contagious viral pathogen affecting dogs aged 6-21 weeks primarily targeting rapidly dividing cells of GI tract, which leads to haemorrhagic gastroenteritis and symptoms such as fever, vomiting, in appetence and abdominal pain. Several variants include CPV-2, CPV-2b and CPV-2c. CPV can be prevented by timely and complete vaccination and can be treated effectively if timely diagnosis is done. It can be diagnosed by effective methods such as PCR, fecal ELISA, HA and HI, the most effective being ELISA and PCR. Therefore, the present research was aimed at developing and standardizing a Double Antibody Sandwich ELISA for the detection of CPV by using rabbit hyperimmune sera as primary antibody and mice hyperimmune sera as secondary antibody.