Peer-reviewed veterinary case report
Development and evaluation of a blocking enzyme-linked immunosorbent assay and virus neutralization assay to detect antibodies to viral hemorrhagic septicemia virus.
- Journal:
- Clinical and vaccine immunology : CVI
- Year:
- 2014
- Authors:
- Wilson, Anna et al.
- Affiliation:
- Department of Pathobiological Sciences · United States
Plain-English summary
Viral hemorrhagic septicemia virus (VHSV) is a serious virus that affects fish and is monitored by various agencies in the U.S. Currently, the usual way to check for this virus involves isolating it, which can be harmful to the fish and only shows if they are currently infected. Researchers have developed two new tests that are safer and quicker: a virus neutralization test and a blocking enzyme-linked immunosorbent assay (ELISA). The virus neutralization test was very specific but only identified about 43% of infections, while the ELISA was able to detect almost all past infections with a high level of accuracy. These new tests are useful for understanding whether fish have been exposed to VHSV in the past without harming them.
Abstract
Viral hemorrhagic septicemia virus (VHSV) is a target of surveillance by many state and federal agencies in the United States. Currently, the detection of VHSV relies on virus isolation, which is lethal to fish and indicates only the current infection status. A serological method is required to ascertain prior exposure. Here, we report two serologic tests for VHSV that are nonlethal, rapid, and species independent, a virus neutralization (VN) assay and a blocking enzyme-linked immunosorbent assay (ELISA). The results show that the VN assay had a specificity of 100% and sensitivity of 42.9%; the anti-nucleocapsid-blocking ELISA detected nonneutralizing VHSV antibodies at a specificity of 88.2% and a sensitivity of 96.4%. The VN assay and ELISA are valuable tools for assessing exposure to VHSV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24429071/