Peer-reviewed veterinary case report
Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2021
- Authors:
- Xiao, Yixin et al.
- Affiliation:
- The First Affiliated Hospital · China
- Species:
- bird
Abstract
The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33618630/