Peer-reviewed veterinary case report
Development and application of a double-antigen sandwich enzyme-linked immunosorbent assay for detection of antibodies to porcine circovirus 2.
- Journal:
- Clinical and vaccine immunology : CVI
- Year:
- 2012
- Authors:
- Ge, Meng et al.
- Affiliation:
- College of Veterinary Medicine · China
Plain-English summary
Researchers have developed a new test called a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to detect antibodies against porcine circovirus 2 (PCV2) in pigs. They tested this new method on blood samples from 394 pigs and found it to be very accurate, with a sensitivity of about 90.6% and a specificity of about 94.0% when compared to other established tests. They also confirmed that 12 pigs known to be free of PCV2 showed no antibodies over five weeks, indicating that the test is reliable. This new ELISA can be completed in under 90 minutes and could help pig farmers make better decisions about managing diseases related to PCV2. Overall, the test appears to work well and could be a valuable tool for the swine industry.
Abstract
A double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the diagnostic sensitivity, specificity, and accuracy of the assay were, respectively, 90.61, 94.02, and 91.62% compared with IIF and 94.38, 95.28, and 94.67% compared with the commercial ELISA kit. Assay of 12 PCV-free pigs over a 5-week period produced only PCV2-negative titers by all 3 methods. These results and the seroprofiles of 4 pig farms obtained by both the commercial ELISA kit and the double-antigen sandwich ELISA indicate that the sandwich ELISA is a reliable method for detection of antibodies to PCV2. Additionally, the method described here permits the use of undiluted test serum samples simultaneously loaded with horseradish peroxidase (HRP)-conjugated antigen into the test well, and the complete test procedure can be performed in less than 90 min. This double-antigen sandwich ELISA should be a useful tool to aid swine industry professionals in deciding the intervention strategies for the control of PCV2-associated diseases.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22815145/