Peer-reviewed veterinary case report
Detection of foot-and-mouth disease virus RNA by reverse transcription loop-mediated isothermal amplification.
- Journal:
- Virology journal
- Year:
- 2011
- Authors:
- Chen, Hao-tai et al.
- Affiliation:
- Lanzhou Veterinary Research Institute · China
Plain-English summary
Researchers have developed a new test that can quickly detect the foot-and-mouth disease virus (FMDV) in animals. This test, called RT-LAMP, can identify the virus's genetic material in just 45 minutes at a steady temperature. It is more sensitive than another common test called RT-PCR and does not confuse FMDV with other similar viruses. In their study, the test successfully identified 84 samples that had FMDV while correctly ruling out 65 samples that did not. This new method shows promise as a fast and easy way to check for FMDV infections.
Abstract
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus. Furthermore, the assay correctly detected 84 FMDV positive samples but not 65 FMDV negative specimens. The result indicated the potential usefulness of the technique as a simple and rapid procedure for the detection of FMDV infection.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22070774/