Peer-reviewed veterinary case report
Detection of Anaplasma platys in dogs using real-time loop-mediated isothermal amplification.
- Journal:
- Veterinary journal (London, England : 1997)
- Year:
- 2014
- Authors:
- Li, Hua-tao et al.
- Affiliation:
- College of Veterinary Medicine · China
- Species:
- dog
Abstract
Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 °C for 30 min. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24508323/