Peer-reviewed veterinary case report
Detection and Discovery of Coronaviruses in Wild Bird Populations.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2020
- Authors:
- Snoeck, Chantal J & Zohari, Siamak
- Affiliation:
- Department of Infection and Immunity
- Species:
- bird
Abstract
Wild birds are natural hosts of multiple microbial agents, including a wide diversity of coronaviruses. Here we describe a pan-Coronavirus detection RT-PCR method to identify those viruses regardless of the coronavirus genus or nature of the specimen. We also describe a protocol using high-throughput sequencing technologies to obtain their entire genome, which overcomes the inherent difficulties of wild bird coronavirus sequencing, that is, their genetic diversity and the lack of virus isolation methods.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/32833202/