Cryptosporidium in Rabbits: A Global Systematic Review and Meta-Analysis of Prevalence, Species/Genotypes Distribution and Zoonotic Significance.

Abstract

BACKGROUND: This systematic review and meta-analysis assessed the global prevalence, species/genotype distribution and zoonotic impact of Cryptosporidium in rabbits. METHODS: A systematic search of PubMed, Scopus and Web of Science was performed for studies from 2000 to 25 October 2024 on Cryptosporidium spp. in rabbits. Data on publication/implementation years, prevalence rates, rabbit types, diagnostics, countries and species/genotypes were collected. A meta-analysis with random-effects models estimated overall prevalence and assessed heterogeneity using the Iindex. A sensitivity analysis evaluated the robustness of the results. RESULTS: This systematic review included 26 studies with 6093 rabbits from 9 countries, revealing a pooled Cryptosporidium spp. prevalence of 9% (95% CI: 6%-13.4%). Three zoonotic species were found in rabbits: Cryptosporidium cuniculus in 18 studies and each of C. parvum and C. andersoni in 1 study. The isolates included 2 genotypes of C. cuniculus (Va, Vb) and 1 genotype of C. parvum (IIc), along with 18 subtypes of C. cuniculus (VaA16, VaA18, VaA31, VbA18, VbA19, VbA21, VbA22, VbA23, VbA24, VbA25, VbA26, VbA28, VbA29, VbA31, VbA32, VbA33, VbA35 and VbA36). Among these, 11 subtypes (VbA19, VbA22-VbA26, VbA28, VbA29 and VbA31-VbA33) are identified as zoonotic. Pet rabbits had the highest Cryptosporidium spp. pooled prevalence at 21.9% (95% CI: 14.7%-31.3%), followed by farmed rabbits at 9.7% (95% CI: 5.1%-17.8%), wild rabbits at 8.8% (95% CI: 4.8%-15.5%) and laboratory rabbits at 1% (95% CI: 0.3%-3.1%), with higher rates noted in Africa and the AFR WHO region. CONCLUSIONS: This study assessed the global distribution of Cryptosporidium spp. in rabbits, highlighting its zoonotic implications. It serves as a key resource for researchers, veterinarians and public health officials for future studies and control strategies.