controls kidney resident macrophage heterogeneity.

Abstract

Kidney macrophages are comprised of both monocyte-derived and tissue resident populations; however, the heterogeneity of kidney macrophages and factors that regulate their heterogeneity are poorly understood. Herein, we performed single cell RNA sequencing (scRNAseq), fate mapping, and parabiosis to define the cellular heterogeneity of kidney macrophages in healthy mice. Our data indicate that healthy mouse kidneys contain four major subsets of monocytes and two major subsets of kidney resident macrophages (KRM) including a population with enrichedexpression, suggesting monocyte origin. Surprisingly, fate mapping data using the newly developedmodel indicate that less than 50% ofKRM are derived from Ly6cmonocytes. Instead, we find thatexpression in KRM reflects their spatial distribution as this cell population is almost exclusively found in the kidney cortex. We also identifiedas a gene that governs cortex specific accumulation ofKRM and show that loss ofKRM reduces the severity of cystic kidney disease in a mouse model where cysts are mainly localized to the kidney cortex. Collectively, our data indicate thatregulates KRM heterogeneity and niche-specific disease progression.