Construction of HPQ-based activatable fluorescent probe for peroxynitrite and its application in ferroptosis and mice model of LPS-induced inflammation.

Abstract

As one of the important members of reactive oxygen species, ONOOplays a crucial role in signal transduction, immune response, and other physiological activities. Aberrant changes in ONOOlevels in the living organism are usually associated with many diseases. Therefore, it is important to establish a highly selective and sensitive method for the determination of ONOOin vivo. Herein, we designed a novel ratio near-infrared fluorescent probe for ONOOby directly conjugating dicyanoisophorone (DCI) to hydroxyphenyl-quinazolinone (HPQ). Surprisingly, HPQD was unaffected by environmental viscosity and responded rapidly to ONOOwithin 40 s. The linear range of ONOOdetection was from 0 μM to 35 μM. Impressively, HPQD did not react with reactive oxygen species and was sensitive to exogenous/endogenous ONOOin live cells. We also investigated the relationship between ONOOand ferroptosis and achieved in vivo diagnosis and efficacy evaluation of mice model of LPS-induced inflammation, which showed promising prospects of HPQD in ONOO-related studies.