Congenital persistent infection with bovine viral diarrhea virus not observed in piglets.

Abstract

This study was designed to determine whether congenital persistent infection occurs in piglets from gilts experimentally inoculated with bovine viral diarrhea virus type 2 (BVDV-2). Six pregnant gilts were divided into 2 groups, infected (= 4), and control (= 2). The gilts were inoculated at 45 days gestation. Piglets were assessed for 35 days following birth with nasal swab and blood sample collections every 72 hours. Reverse transcriptase-polymerase chain reaction (RT-PCR) tests were performed for direct diagnosis of virus in blood and nasal swabs, and virus neutralization was used for antibody detection. Transplacental transmission of BVDV-2 did not occur. Piglets were born free of the virus and did not shed BVDV during the experimental period.