Peer-reviewed veterinary case report
Complete genome sequence analysis of Japanese encephalitis virus isolated from a horse in India.
- Journal:
- Archives of virology
- Year:
- 2013
- Authors:
- Singha, Harisankar et al.
- Affiliation:
- Equine Health Unit · India
- Species:
- horse
Plain-English summary
Researchers studied a strain of the Japanese encephalitis virus (JEV) taken from a horse in India. They found that the virus's genetic material is quite similar to other known strains, showing a high level of similarity in its DNA and protein sequences. This particular strain, called H225, is classified as highly virulent, meaning it has the potential to cause serious illness. This study is important because it is the first time a complete genome of this specific type of JEV has been sequenced from a horse. Overall, the findings highlight the potential risks associated with this virus in horses.
Abstract
The complete genome of the Japanese encephalitis virus (JEV) strain JEV/eq/India/H225/2009(H225), isolated from an infected horse in India, was sequenced and compared to previously published JEV genomes. H225 genome was 10,977-nucleotides long, comprising a single ORF of 10,299-nucleotides, a 5'-UTR of 95 nucleotides and a 3'-UTR of 582 nucleotides. The H225 genome showed high levels of sequence identity with 47 fully sequenced JEV genomes, ranging from 99.3 % to 75.5 % for nucleotides and 99.2 % to 91.5 % for amino acid sequences. Phylogenetic analysis of the full-length sequence indicated that the H225 strain belongs to genotype III and is closely related to the Indian JEV strain Vellore P20778. A comparison of amino acids associated with neurovirulence in the E proteins and non-structural proteins of known virulent and attenuated JEV strains suggested H225 to be a highly virulent strain. This is the first report of whole-genome sequencing of a genotype III JEV genome isolated from equines.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23001697/