Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma.

Abstract

BACKGROUND: To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP). OBJECTIVES: We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing. METHODS: Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (-20&#xb0;C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24&#x2009;h and one refrozen for 7&#x2009;days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO. RESULTS: FVIII activity was higher in LR-CRYO (p&#x2009;=&#x2009;0.0001) versus LR-FP. vWF activity (p&#x2009;<&#x2009;0.0001) and fibrinogen concentration (p&#x2009;=&#x2009;0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (p&#x2009;<&#x2009;0.0001) and LR-FP (p&#x2009;=&#x2009;0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (p&#x2009;<&#x2009;0.0001) and LR-CRYO (p&#x2009;<&#x2009;0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (p&#x2009;=&#x2009;0.0062) in refrigerated LR-CRYO. CONCLUSIONS: Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.