Peer-reviewed veterinary case report
Comparison of a loop-mediated isothermal amplification for orf virus with quantitative real-time PCR.
- Journal:
- Virology journal
- Year:
- 2013
- Authors:
- Wang, Guangxiang et al.
- Affiliation:
- State Key Laboratory of Veterinary Etiological Biology · China
Plain-English summary
Orf virus causes a serious skin disease called orf, which affects goats, sheep, and other similar animals. Researchers developed a new test called loop-mediated isothermal amplification (LAMP) to quickly and accurately detect this virus. They found that this test was very sensitive and specific, meaning it could reliably identify infections without confusing them with other viruses. The LAMP test performed almost as well as the traditional real-time PCR test, showing a high agreement in results. Overall, the LAMP method is a promising new tool for diagnosing orf virus infections in livestock.
Abstract
BACKGROUND: Orf virus (ORFV) causes orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. Therefore, a rapid, highly specific and accurate method for the diagnosis of ORFV infections is essential to ensure that the appropriate treatments are administered and to reduce economic losses. METHODS: A loop-mediated isothermal amplification (LAMP) assay based on the identification of the F1L gene was developed for the specific detection of ORFV infections. The sensitivity and specificity of the LAMP assay were evaluated, and the effectiveness of this method was compared with that of real-time PCR. RESULTS: The sensitivity of this assay was determined to be 10 copies of a standard plasmid. Furthermore, no cross-reactivity was found with either capripox virus or FMDV. The LAMP and real-time PCR assays were both able to detect intracutaneous- and cohabitation-infection samples, with a concordance of 97.83%. LAMP demonstrated a sensitivity of 89.13%. CONCLUSION: The LAMP assay is a highly efficient and practical method for detecting ORFV infection. This LAMP method shows great potential for monitoring the prevalence of orf, and it could prove to be a powerful supplemental tool for current diagnostic methods.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23634981/