Coagulation factor and hemostatic protein content of canine plasma after storage of whole blood at ambient temperature.

Abstract

BACKGROUND: Standard practice in canine blood banking is to produce fresh frozen plasma (FFP) by separating and freezing plasma produced from blood within 8&#xa0;hours of collection. Within canine blood donation programs, this can limit the number of units collected. HYPOTHESIS/OBJECTIVES: The aim was to compare the coagulation factor and hemostatic protein content (CF&HPC) of plasma produced from blood stored at ambient temperature for 8, 12, and 24&#xa0;hours. Another aim was to compare the CF&HPC between Greyhound types and other breeds. ANIMALS: None. METHODS: In vitro study. A convenience sample of 58 units of canine blood from a blood donor pool was processed to prepare and freeze plasma 8, 12, or 24&#xa0;hours following collection. RESULTS: Regardless of time of processing, the units contained therapeutic CF&HPC. Frozen plasma prepared after 24&#xa0;hours had significantly higher factor VIII (P&#xa0;=&#xa0;.014) and factor X (P&#xa0;=&#xa0;.03) when compared with the frozen plasma prepared at 8&#xa0;hours. Factor X (P&#xa0;<&#xa0;.01), fibrinogen (P&#xa0;<&#xa0;.01), and vWF (P&#xa0;=&#xa0;.04) were significantly lower in plasma collected from Greyhound types than in plasma collected from other breeds. CONCLUSIONS AND CLINICAL IMPORTANCE: Storing whole blood for up to 24&#xa0;hours is a suitable method for producing FFP. Lower values for some coagulation factors and hemostatic proteins in plasma produced from Greyhound types would not preclude these dogs as FFP donors.