Clinical Observations After Prebreeding Intrauterine Plasma Infusion in 18 Mares Inseminated With Thawed Frozen Semen.

Abstract

The use of autologous blood processing tools including platelet-rich plasma (PRP) devices is increasingly widespread in veterinary medicine. In equine reproduction, a number of studies have explored the effects of intrauterine infusion of PRP on persistent mating-induced endometritis. Artificial insemination with thawed frozen semen incites an intrauterine inflammatory response and we sought to extend the applications of intrauterine PRP to normal mares being inseminated with frozen semen. We investigated a subset of our normal breeding population to observe the clinical effects of prebreeding intrauterine infusion of PRP and plasma followed by insemination with frozen semen. Eighteen mares were treated after failure to achieve pregnancy after one breeding cycle of artificial insemination with frozen semen. Breeding management was performed in a routine manner, and each mare was bred to the same stallion as the previous cycle. Autologous PRP was prepared using a commercial platelet isolation device; PRP was diluted with Platelet-poor Plasma, and intrauterine infusion was performed 12-48 hours before artificial insemination. This case series represents an initial investigation into the clinical effects of intrauterine PRP and plasma infused before insemination with thawed frozen semen in a population of clinically normal mares.