Peer-reviewed veterinary case report
Characterization of an equine macrophage cell line: application to studies of EIAV infection.
- Journal:
- Veterinary microbiology
- Year:
- 2009
- Authors:
- Fidalgo-Carvalho, Isabel et al.
- Affiliation:
- Iron Genes and the Immune System
- Species:
- horse
Abstract
EIAV is a monocyte/macrophage tropic virus. To date, even though EIAV has been under investigation for numerous years, very few details have been elucidated about EIAV/macrophage interactions. This is largely due to the absence of an equine macrophage cell line that would support viral replication. Herein we describe the spontaneous immortalization and generation of a clonal equine macrophage-like (EML) cell line with the functional and immunophenotype characteristics of differentiated equine monocyte derived macrophage(s) (eMDM(s)). These cells possess strong non-specific esterase (NSE) activity, are able to phagocytose fluorescent bioparticles, and produce nitrites in response to LPS. The EML-3C cell line expresses the EIAV receptor for cellular entry (ELR1) and supports replication of the virulent EIAV(PV) biological clone. Thus, EML-3C cells provide a useful cell line possessing equine macrophage related properties for the growth and study of EIAV infection as well as of other equine macrophage tropic viruses.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19038510/