BW2013 protects mucosal integrity and modulates gut microbiota of mice with colitis.

Abstract

This study explored the effects of(previously) BW2013 on mucosal integrity and gut microbiota of mice with dextran sulfate sodium (DSS)-induced colitis. The results show that the clinical symptoms in DSS-modelled ulcerative colitis (UC) were improved byBW2013 via decreasing disease activity index scores and suppressing inflammatory cell infiltration. Furthermore,BW2013 decreased the levels of diamine oxidase activity, myeloperoxidase, and D-lactic acid. The mRNA expression of ZO-1, occludin, and claudin-1 was upregulated byBW2013, which also increased IL-10 and reduced TNF-α, IL-1β, and IL-6 in the colon. 16S rDNA sequencing showed thatBW2013 enhanced α-diversity.BW2013 upregulated significantly the abundance of unidentfied,,,, and, which had an inhibitory effect on inflammation and a protective effect on the integrity of the mucosa. These results demonstrate thatBW2013 alleviates DSS-modelled UC by protecting mucosal integrity and ameliorating the composition of gut microbiota.