An Obstructive Chronic Pancreatitis Model Established Through Electrocoagulation.

Abstract

Chronic pancreatitis (CP) is a severe inflammatory condition that can destroy the pancreas, lead to diabetes, and increase pancreatic cancer risk, severely impacting patients' quality of life. Addressing CP is a critical research focus in gastroenterology. We developed a new animal model to overcome the limitations of existing ones, using 8-week-old C57BL/6 male mice. After exposing the pancreas through abdominal surgery on anesthetized mice, we clamped the common bile duct near the liver to prevent methylene blue from entering it. A capillary was inserted into the bile duct to inject the dye, and after the pancreatic duct was stained. After the pancreatic duct had been visualized, the pancreas was selected for electrocoagulation in the middle and lower 2/3 of the position between the common bile duct and the superior pancreaticoduodenal artery. Electrocoagulation was terminated when the blue staining solution could not be recognized. The abdomen was then closed, and the mice were normally raised. At 7, 14, 21, and 28 days post surgery, the pancreas were examined using HE staining, Masson staining, and immunohistochemistry, and blood serum was analyzed for biochemical parameters. The pathology after 14 days closely matched the characteristics of chronic pancreatitis, as did the serum biochemical parameters.