A two-photon fluorescent probe for sensitive detection and imaging of hypochlorite in depression model.

Abstract

Hypochlorite (ClO) is involved in numerous physiological and pathological processes. Real-time monitoring of ClOlevels is essential for understanding its role in diseases. In this study, a novel selenide-based two-photon fluorescent probe Cou-Se was developed for ClOdetection based on coumarin fluorophore and 2-phenylseleno acetate. Cou-Se exhibits a selective and sensitive fluorescence turn-on response to ClOpeaked at 455 nm. The detection limit toward ClOwas determined to be 0.09 μM. Cou-Se was successfully used to image exogenous and endogenous ClOin living cells. One-photon and two-photon fluorescence imaging of Cou-Se reveals significantly higher levels of ClOin CORT-induced depressive PC12 cells when compare to normal cells. Brain tissue of depressive mice also exhibited elevated levels of ClOthrough two-photon fluorescence imaging. Our work offers a promising tool for advancing diagnostic and therapeutic research on depression.