Peer-reviewed veterinary case report
A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2012
- Authors:
- Wang, Shuai et al.
- Affiliation:
- Institute of Animal Science · China
Abstract
Three Holstein sires were identified as complex vertebral malformation (CVM) carriers using the polymerase chain reaction-primer introduced restriction analysis (PCR-PIRA) method. Using the carriers as positive controls, the PCR mismatch amplification mutation assay (MAMA PCR) method was developed and validated by sequence analysis. With MAMA PCR, 154 Chinese Holstein sires were tested for CVM, among which 24 were confirmed to be CVM carriers. With DNA isolated from hair follicles, 10 daughters of one CVM-positive bull were detected, among which 7 were confirmed to be CVM carriers.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22529127/