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Peer-reviewed veterinary case report

A longitudinal Raman microspectroscopic study of osteoporosis induced by spinal cord injury.

Journal:
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
Year:
2010
Authors:
Shen, J et al.
Affiliation:
College of Chemistry and Molecular Sciences · China
Species:
rodent

Abstract

INTRODUCTION: A longitudinal study was established to investigate bone compositional information in spinal cord injury (SCI) rat model. METHODS: Raman spectroscopy was applied to detect the distal femur and humeri of SCI, sham-operated (SO), and age-matched control (CON) male Sprague-Dawley (SD) rats at first, second, third, and fifth weeks after surgery. One-way ANOVA and Tukey's HSD post hoc multiple comparison tests were used to analyze the longitudinal data of mineral to matrix ratio and carbonate substitution. RESULTS: Relative mineral decrease was found in SCI group by more than 20% in femur and approximately 12% in humeri compared with CON group. No significant changes in carbonate substitution were observed. CONCLUSIONS: Severe bone loss in the early stage of SCI was confirmed by a continuous decrease of the mineral to collagen matrix ratio. The decrease in the humeri suggested hormone level variations might participate in the etiology of SCI-induced osteoporosis.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19436936/