Peer-reviewed veterinary case report
2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2018
- Authors:
- Guest, Paul C
- Affiliation:
- Department of Biochemistry and Tissue Biology · Brazil
- Species:
- rodent
Abstract
This chapter describes the basics of two-dimensional difference gel electrophoresis (2D-DIGE) for multiplex analysis of two distinct proteomes. The example given describes the analysis of male and female rat lens soluble proteins labeled with fluorescent Cy3 and Cy5 dyes in comparison to a pooled standard labeled with Cy2. After labeling the proteomes are mixed together and electrophoresed on the same 2D gels. Scanning the gels at wavelengths specific for each dye allows direct overlay the two different proteomes. Differences in abundance of specific protein spots can be determined through comparison to the pooled standard.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/29380333/